abstract：:Glucagon-like peptide-1 receptor (GLP-1R) agonists used to treat type 2 diabetes mellitus often produce nausea, vomiting, and in some patients, undesired anorexia. Notably, these behavioral effects are caused by direct central GLP-1R activation. Herein, we describe the creation of a GLP-1R agonist conjugate with modif...

journal_title：Cell reports

authors： Borner T,Workinger JL,Tinsley IC,Fortin SM,Stein LM,Chepurny OG,Holz GG,Wierzba AJ,Gryko D,Nexø E,Shaulson ED,Bamezai A,Da Silva VAR,De Jonghe BC,Hayes MR,Doyle RP

更新日期：2020-06-16 00:00:00

abstract：:Ischemic stroke is a well-recognized disease of aging, yet it is unclear how the age-dependent vulnerability occurs and what are the underlying mechanisms. To address these issues, we perform a comprehensive RNA-seq analysis of aging, ischemic stroke, and their interaction in 3- and 18-month-old mice. We assess differ...

journal_title：Cell reports

authors： Androvic P,Kirdajova D,Tureckova J,Zucha D,Rohlova E,Abaffy P,Kriska J,Valny M,Anderova M,Kubista M,Valihrach L

更新日期：2020-06-16 00:00:00

abstract：:Kainate receptors (KARs) mediate postsynaptic currents with a key impact on neuronal excitability. However, the molecular determinants controlling KAR postsynaptic localization and stabilization are poorly understood. Here, we exploit optogenetic and single-particle tracking approaches to study the role of KAR conform...

journal_title：Cell reports

authors： Polenghi A,Nieus T,Guazzi S,Gorostiza P,Petrini EM,Barberis A

更新日期：2020-06-09 00:00:00

abstract：:Spatiotemporal protein reorganization at DNA damage sites induced by genotoxic chemotherapies is crucial for DNA damage response (DDR), which influences treatment response by directing cancer cell fate. This process is orchestrated by valosin-containing protein (VCP), an AAA+ ATPase that extracts polyubiquinated chrom...

journal_title：Cell reports

authors： Zhu C,Rogers A,Asleh K,Won J,Gao D,Leung S,Li S,Vij KR,Zhu J,Held JM,You Z,Nielsen TO,Shao J

更新日期：2020-06-09 00:00:00

abstract：:Neurons face unique transport challenges. They need to deliver cargo over long axonal distances and to many presynaptic nerve terminals. Rab GTPases are master regulators of vesicular traffic, but essential presynaptic Rabs have not been identified. Here, we find that Rab6, a Golgi-derived GTPase for constitutive secr...

journal_title：Cell reports

authors： Nyitrai H,Wang SSH,Kaeser PS

更新日期：2020-06-09 00:00:00

abstract：:Fast purinergic signaling is mediated by ATP and ATP-gated ionotropic P2X receptors (P2XRs), and it is implicated in pain-related behaviors. The properties exhibited by P2XRs vary between those expressed in heterologous cells and in vivo. Several modulators of ligand-gated ion channels have recently been identified, s...

journal_title：Cell reports

authors： Salm EJ,Dunn PJ,Shan L,Yamasaki M,Malewicz NM,Miyazaki T,Park J,Sumioka A,Hamer RRL,He WW,Morimoto-Tomita M,LaMotte RH,Tomita S

更新日期：2020-06-02 00:00:00

abstract：:Here, we propose an approach to identify active metabolic pathways by integrating gene essentiality analysis and protein abundance. We use two bacterial species (Mycoplasma pneumoniae and Mycoplasma agalactiae) that share a high gene content similarity yet show significant metabolic differences. First, we build detail...

journal_title：Cell reports

authors： Montero-Blay A,Piñero-Lambea C,Miravet-Verde S,Lluch-Senar M,Serrano L

更新日期：2020-06-02 00:00:00

abstract：:As three-dimensional cell culture formats gain in popularity, there emerges a need for tools that produce vast amounts of data on individual cells within the spheroids or organoids. Here, we present a microfluidic platform that provides access to such data by parallelizing the manipulation of individual spheroids with...

journal_title：Cell reports

authors： Tomasi RF,Sart S,Champetier T,Baroud CN

更新日期：2020-05-26 00:00:00

abstract：:The flat bones of the skull are densely innervated during development, but little is known regarding their role during repair. We describe a neurotrophic mechanism that directs sensory nerve transit in the mouse calvaria. Patent cranial suture mesenchyme represents an NGF (nerve growth factor)-rich domain, in which se...

journal_title：Cell reports

authors： Meyers CA,Lee S,Sono T,Xu J,Negri S,Tian Y,Wang Y,Li Z,Miller S,Chang L,Gao Y,Minichiello L,Clemens TL,James AW

更新日期：2020-05-26 00:00:00

abstract：:Generation of insulin-secreting β cells in vitro is a promising approach for diabetes cell therapy. Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are differentiated to β cells (SC-β cells) and mature to undergo glucose-stimulated insulin secretion, but molecular regulation of thi...

journal_title：Cell reports

authors： Velazco-Cruz L,Goedegebuure MM,Maxwell KG,Augsornworawat P,Hogrebe NJ,Millman JR

更新日期：2020-05-26 00:00:00

abstract：:CRISPR genome engineering has become a powerful tool to functionally investigate the complex mechanisms of immune system regulation. While decades of work have aimed to genetically reprogram innate immunity, the utility of current approaches is restricted by poor knockout efficiencies or limited specificity for mature...

journal_title：Cell reports

authors： Riggan L,Hildreth AD,Rolot M,Wong YY,Satyadi W,Sun R,Huerta C,O'Sullivan TE

更新日期：2020-05-19 00:00:00

abstract：:We report the emergence of an endogenous circadian clock that regulates organogenesis in mouse fetal kidney. We detect circadian rhythms both in vivo with transcriptional profiling and ex vivo by bioluminescence. High-resolution structural analysis of embryonic explants reveals that global or local clock disruption re...

journal_title：Cell reports

authors： Dan H,Ruan T,Sampogna RV

更新日期：2020-05-19 00:00:00

abstract：:The enteroinvasive bacterium Shigella flexneri forces its uptake into non-phagocytic host cells through the translocation of T3SS effectors that subvert the actin cytoskeleton. Here, we report de novo actin polymerization after cellular entry around the bacterium-containing vacuole (BCV) leading to the formation of a ...

journal_title：Cell reports

authors： Kühn S,Bergqvist J,Gil M,Valenzuela C,Barrio L,Lebreton S,Zurzolo C,Enninga J

更新日期：2020-05-12 00:00:00

abstract：:Whereas hundreds of cells in the mouse embryonic aorta transdifferentiate to hematopoietic cells, only very few establish hematopoietic stem cell (HSC) identity at a single time point. The Gata2 transcription factor is essential for HSC generation and function. In contrast to surface-marker-based cell isolation, Gata2...

journal_title：Cell reports

authors： Vink CS,Calero-Nieto FJ,Wang X,Maglitto A,Mariani SA,Jawaid W,Göttgens B,Dzierzak E

更新日期：2020-05-12 00:00:00

abstract：:Polyunsaturated fatty acids (PUFAs) confer health benefits by preventing inflammation and obesity and by increasing thermogenesis in brown and beige adipocytes. As well as being supplied exogenously as nutrients, PUFAs are largely stored in membrane glycerophospholipids and released by phospholipase A2s (PLA2s). Howev...

journal_title：Cell reports

authors： Sato H,Taketomi Y,Miki Y,Murase R,Yamamoto K,Murakami M

更新日期：2020-05-05 00:00:00

abstract：:Eukaryotic genomes are almost entirely transcribed by RNA polymerase II. Consequently, the transcription of long noncoding RNAs often overlaps with coding gene promoters, triggering potential gene repression through a poorly characterized mechanism of transcription interference. Here, we propose a comprehensive model ...

journal_title：Cell reports

authors： Gill JK,Maffioletti A,García-Molinero V,Stutz F,Soudet J

更新日期：2020-05-05 00:00:00

abstract：:Attempts to generate functional blood cells from human pluripotent stem cells (hPSCs) remain largely unsuccessful, mainly due to the lack of understanding of the regulatory network of human hematopoiesis. In this study, we identified leucine-rich-repeat-containing G-protein-coupled receptor 4 (LGR4) as an essential re...

journal_title：Cell reports

authors： Wang Y,Wang H,Guo J,Gao J,Wang M,Xia M,Wen Y,Su P,Yang M,Liu M,Shi L,Cheng T,Zhou W,Zhou J

更新日期：2020-05-05 00:00:00

abstract：:Nutrient transporters can be rapidly removed from the cell surface via substrate-stimulated endocytosis as a way to control nutrient influx, but the molecular underpinnings are not well understood. In this work, we focus on zinc-dependent endocytosis of human ZIP4 (hZIP4), a zinc transporter that is essential for diet...

journal_title：Cell reports

authors： Zhang C,Sui D,Zhang T,Hu J

更新日期：2020-04-28 00:00:00

abstract：:Zika virus (ZIKV) is an emerging, mosquito-borne flavivirus responsible for recent epidemics across the Americas, and it is closely related to dengue virus (DENV). Here, we study samples from 46 DENV-naive and 43 DENV-immune patients with RT-PCR-confirmed ZIKV infection at early-acute, late-acute, and convalescent tim...

journal_title：Cell reports

authors： Michlmayr D,Kim EY,Rahman AH,Raghunathan R,Kim-Schulze S,Che Y,Kalayci S,Gümüş ZH,Kuan G,Balmaseda A,Kasarskis A,Wolinsky SM,Suaréz-Fariñas M,Harris E

更新日期：2020-04-28 00:00:00

abstract：:Investigating mechanisms that regulate endothelial cell (EC) growth and survival is important for understanding EC homeostasis and how ECs maintain stem cell niches. We report here that targeted loss of Id genes in adult ECs results in dilated, leaky sinusoids and a pro-inflammatory state that increases in severity ov...

journal_title：Cell reports

authors： Gadomski S,Singh SK,Singh S,Sarkar T,Klarmann KD,Berenschot M,Seaman S,Jakubison B,Gudmundsson KO,Lockett S,Keller JR

更新日期：2020-04-28 00:00:00

abstract：:The anorectic and weight-suppressive effects of growth differentiation factor-15 (GDF15) are attracting considerable attention for treating obesity. Current experiments in rats investigate whether GDF15 induces an aversive visceral malaise-based state that mediates its acute anorectic effect and, through aversion cond...

journal_title：Cell reports

authors： Borner T,Wald HS,Ghidewon MY,Zhang B,Wu Z,De Jonghe BC,Breen D,Grill HJ

更新日期：2020-04-21 00:00:00

abstract：:The asymmetric distribution of auxin leads to the bending growth of hypocotyls during gravitropic and phototropic responses, but the signaling events downstream of auxin remain unclear. Here, we identify many SAUR genes showing asymmetric expression in soybean hypocotyls during gravistimulation and then study their ho...

journal_title：Cell reports

authors： Wang X,Yu R,Wang J,Lin Z,Han X,Deng Z,Fan L,He H,Deng XW,Chen H

更新日期：2020-04-21 00:00:00

abstract：:Cell growth is coupled to cell-cycle progression in mitotically proliferating mammalian cells, but the underlying molecular mechanisms are not well understood. CyclinD-Cdk4/6 is known to phosphorylate RB to promote S-phase entry, but recent work suggests they have additional functions. We show here that CyclinD-Cdk4/6...

journal_title：Cell reports

authors： Romero-Pozuelo J,Figlia G,Kaya O,Martin-Villalba A,Teleman AA

更新日期：2020-04-14 00:00:00

abstract：:The metabolic enzyme phosphoglycerate mutase 1 (PGAM1) is overexpressed in several types of cancer, suggesting an additional function beyond its established role in the glycolytic pathway. We here report that PGAM1 is overexpressed in gliomas where it increases the efficiency of the DNA damage response (DDR) pathway b...

journal_title：Cell reports

authors： Ohba S,Johannessen TA,Chatla K,Yang X,Pieper RO,Mukherjee J

更新日期：2020-04-14 00:00:00

abstract：:Medulloblastoma (MB) is a neoplasm linked to dysregulated cerebellar development. Previously, we demonstrated that the Sonic Hedgehog (SHH) subgroup grows hierarchically, with Sox2+ cells at the apex of tumor progression and relapse. To test whether this mechanism is rooted in a normal developmental process, we studie...

journal_title：Cell reports

authors： Selvadurai HJ,Luis E,Desai K,Lan X,Vladoiu MC,Whitley O,Galvin C,Vanner RJ,Lee L,Whetstone H,Kushida M,Nowakowski T,Diamandis P,Hawkins C,Bader G,Kriegstein A,Taylor MD,Dirks PB

更新日期：2020-04-14 00:00:00

abstract：:High-throughput single-cell RNA sequencing (scRNA-seq) has become a frequently used tool to assess immune cell heterogeneity. Recently, the combined measurement of RNA and protein expression was developed, commonly known as cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq). Acquisition of prote...

journal_title：Cell reports

authors： Mair F,Erickson JR,Voillet V,Simoni Y,Bi T,Tyznik AJ,Martin J,Gottardo R,Newell EW,Prlic M

更新日期：2020-04-07 00:00:00

abstract：:Stimulator of Interferon Genes (STING) is a critical component of host innate immune defense but can contribute to chronic autoimmune or autoinflammatory disease. Once activated, the cyclic guanosine monophosphate (GMP)-adenosine monophosphate (AMP) (cGAMP) synthase (cGAS)-STING pathway induces both type I interferon ...

journal_title：Cell reports

authors： Balka KR,Louis C,Saunders TL,Smith AM,Calleja DJ,D'Silva DB,Moghaddas F,Tailler M,Lawlor KE,Zhan Y,Burns CJ,Wicks IP,Miner JJ,Kile BT,Masters SL,De Nardo D

更新日期：2020-04-07 00:00:00

abstract：:The cytokine interleukin-1β (IL-1β) is critical for antimicrobial defenses; the inflammasome pathway typically controls IL-1β release, but pathogens often evade this pathway. In this issue, Donado et al. (2020) describe an alternative, two-cell model, to instruct inflammasome-independent IL-1β release. ...

journal_title：Cell reports

authors： Bezbradica JS,Joyce S

更新日期：2020-04-07 00:00:00

abstract：:Cellular translation surveillance rescues ribosomes that stall on problematic mRNAs. During translation surveillance, endonucleolytic cleavage of the problematic mRNA is a critical step in rescuing stalled ribosomes. Here we identify NONU-1 as a factor required for translation surveillance pathways including no-go and...

journal_title：Cell reports

authors： Glover ML,Burroughs AM,Monem PC,Egelhofer TA,Pule MN,Aravind L,Arribere JA

更新日期：2020-03-31 00:00:00

abstract：:Silencing of FMR1 and loss of its gene product, FMRP, results in fragile X syndrome (FXS). FMRP binds brain mRNAs and inhibits polypeptide elongation. Using ribosome profiling of the hippocampus, we find that ribosome footprint levels in Fmr1-deficient tissue mostly reflect changes in RNA abundance. Profiling over a t...

journal_title：Cell reports

authors： Shah S,Molinaro G,Liu B,Wang R,Huber KM,Richter JD

更新日期：2020-03-31 00:00:00

abstract：:Macrophages exist predominantly in two distinct states, G0 and a G1-like state that is accompanied by phosphorylation of SAMHD1 at T592. Here, we demonstrate that Toll-like receptor 4 (TLR4) activation can potently induce G0 arrest and SAMHD1 antiretroviral activity by an interferon (IFN)-independent pathway. This pat...

journal_title：Cell reports

authors： Mlcochova P,Winstone H,Zuliani-Alvarez L,Gupta RK

更新日期：2020-03-24 00:00:00

abstract：:Systemic immune dysregulation contributes to the development of neuropsychiatric and neurodegenerative diseases. The precise effect of chronic peripheral immune stimulation on myeloid cells across anatomical brain regions is unclear. Here, we demonstrate brain-region-specific differences in myeloid responses induced b...

journal_title：Cell reports

authors： Süß P,Hoffmann A,Rothe T,Ouyang Z,Baum W,Staszewski O,Schett G,Prinz M,Krönke G,Glass CK,Winkler J,Schlachetzki JCM

更新日期：2020-03-24 00:00:00

abstract：:A mature olfactory sensory neuron (OSN) of the main olfactory epithelium (MOE) typically expresses one allele of one odorant receptor (OR) gene. It is widely thought that the great majority of the 1,141 intact mouse OR genes are expressed in one of four MOE zones (or bands or stripes), which are largely non-overlappin...

journal_title：Cell reports

authors： Zapiec B,Mombaerts P

更新日期：2020-03-24 00:00:00

abstract：:Infections can result in a temporarily restricted unresponsiveness of the innate immune response, thereby limiting pathogen control. Mechanisms of such unresponsiveness are well studied in lipopolysaccharide tolerance; however, whether mechanisms of tolerance limit innate immunity during virus infection remains unknow...

journal_title：Cell reports

authors： Adomati T,Cham LB,Hamdan TA,Bhat H,Duhan V,Li F,Ali M,Lang E,Huang A,Naser E,Khairnar V,Friedrich SK,Lang J,Friebus-Kardash J,Bergerhausen M,Schiller M,Machlah YM,Lang F,Häussinger D,Ferencik S,Hardt C,Lang PA,

更新日期：2020-03-17 00:00:00

abstract：:DC-SIGN+ monocyte-derived dendritic cells (mo-DCs) play important roles in bacterial infections and inflammatory diseases, but the factors regulating their differentiation and proinflammatory status remain poorly defined. Here, we identify a microRNA, miR-181a, and a molecular mechanism that simultaneously regulate th...

journal_title：Cell reports

authors： Lim CX,Lee B,Geiger O,Passegger C,Beitzinger M,Romberger J,Stracke A,Högenauer C,Stift A,Stoiber H,Poidinger M,Zebisch A,Meister G,Williams A,Flavell RA,Henao-Mejia J,Strobl H

更新日期：2020-03-17 00:00:00

abstract：:Our knowledge of transcriptional heterogeneities in epithelial stem and progenitor cell compartments is limited. Epidermal basal cells sustain cutaneous tissue maintenance and drive wound healing. Previous studies have probed basal cell heterogeneity in stem and progenitor potential, but a comprehensive dissection of ...

journal_title：Cell reports

authors： Haensel D,Jin S,Sun P,Cinco R,Dragan M,Nguyen Q,Cang Z,Gong Y,Vu R,MacLean AL,Kessenbrock K,Gratton E,Nie Q,Dai X

更新日期：2020-03-17 00:00:00

abstract：:Changes in epigenetic states affect organismal homeostasis, including stress resistance. However, the mechanisms coordinating epigenetic states and systemic stress resistance remain largely unknown. Here, we identify the intestine-to-germline communication of epigenetic states, which intergenerationally enhances stres...

journal_title：Cell reports

authors： Nono M,Kishimoto S,Sato-Carlton A,Carlton PM,Nishida E,Uno M

更新日期：2020-03-10 00:00:00

abstract：:Eukaryotic DNA replication licensing is a prerequisite for, and plays a role in, regulating genome duplication that occurs exactly once per cell cycle. ORC (origin recognition complex) binds to and marks replication origins throughout the cell cycle and loads other replication-initiation proteins onto replication orig...

journal_title：Cell reports

authors： Amin A,Wu R,Cheung MH,Scott JF,Wang Z,Zhou Z,Liu C,Zhu G,Wong CK,Yu Z,Liang C

更新日期：2020-03-10 00:00:00

abstract：:Astroglia regulate neurovascular coupling while engaging in signal exchange with neurons. The underlying cellular machinery is thought to rely on astrocytic Ca2+ signals, but what controls their amplitude and waveform is poorly understood. Here, we employ time-resolved two-photon excitation fluorescence imaging in acu...

journal_title：Cell reports

authors： King CM,Bohmbach K,Minge D,Delekate A,Zheng K,Reynolds J,Rakers C,Zeug A,Petzold GC,Rusakov DA,Henneberger C

更新日期：2020-03-10 00:00:00

abstract：:Memory B cells (MBCs) epitomize the adaptation of the immune system to the environment. We identify two MBC subsets in peripheral blood, CD27dull and CD27bright MBCs, whose frequency changes with age. Heavy chain variable region (VH) usage, somatic mutation frequency replacement-to-silent ratio, and CDR3 property chan...

journal_title：Cell reports

authors： Grimsholm O,Piano Mortari E,Davydov AN,Shugay M,Obraztsova AS,Bocci C,Marasco E,Marcellini V,Aranburu A,Farroni C,Silvestris DA,Cristofoletti C,Giorda E,Scarsella M,Cascioli S,Barresi S,Lougaris V,Plebani A,Cancrini C

更新日期：2020-03-03 00:00:00